Method for efficiently separating and purifying mammary epithelial cells

一种高效分离纯化乳腺上皮细胞的方法

Abstract

The invention discloses a method for efficiently separating and purifying mammary epithelial cells. The method is suitable for a plurality of mammals. The mammary tissues of adult mammals, which are in the middle and later periods of gestation or in the lactation period, are collected through aseptic operation. According to the provided method, the separation and purification can be achieved in one step, the whole experiment is simple and efficient; the purification, which comprises repeated differential digestion and differential attachment, in the conventional method is eliminated, the purity of mammary epithelial cells obtained by the provided method can reach 98% or more, the obtained mammary epithelial cells can be continuously cultured in-vitro and passed down for 40 generations, and the lactating function of mammary epithelial cells is not affected. The obtained mammary epithelial cells can be applied to the researches on growth of mammary gland, lactating mechanism, mammary bioreactor, transgenic animals, mammary diseases, and the like.
本发明公开了一种高效分离纯化乳腺上皮细胞的方法,适用于多种哺乳动物。本方法通过无菌手术采集妊娠中后期或泌乳期成年哺乳动物的乳腺组织,使用发明的方法,一步操作即可达到分离纯化目的,整个实验简单高效,省去了传统方法中反复差速消化和差速贴壁的纯化过程,通过本方法获得的乳腺上皮细胞纯度达到98%以上,并可在体外连续培养传代40代以上,不影响乳腺上皮细胞的泌乳功能。本发明的细胞可用于乳腺生长发育、泌乳机制、乳腺生物反应器、转基因动物、乳腺疾病等机理的研究。

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