A making method of a mulberry fruit wine is a method of making a fruit wine by steps of preparing a mulberry juice, directly fermenting, modulating and fermenting. The method abandons a traditional fruit wine making method of distillation after fermentation. Thus, a lot of energy sources required by distillation can be saved. On the other hand, as the fruit wine is prepared directly from the mulberry juice, active ingredients of mulberry are maintained in the prepared fruit wine, trace elements contained in mulberry are maintained and specific fragrance of mulberry is maintained. Thus, the fruit wine prepared by the method has high nutritive value and good mouthfeel in comparison with a fruit wine prepared by a previous fruit wine making method, and the mulberry fruit wine is beneficial to people's body health.