临床治疗级细胞治疗用毛囊干细胞细胞外基质筛选培养方法

Method for screening and culturing extracellular hair follicle stem cell matrix for clinic treatment level cell therapy

Abstract

本发明公开了临床治疗级细胞治疗用毛囊干细胞的培养、筛选、扩增技术,这些瓶颈一直是生物学干细胞领域研究热点和难点,目前利用基底膜显著黏附特性进行分离纯化,采用三维培养扩增手段获得。该发明适合依赖扩增贴壁型细胞并提供三维高拟体内细胞外基质系统进行目标细胞水平的筛选与分离。高模拟体内贴附环境,体外培养成体(胚胎皮肤)干细胞的生物细胞新培养技术。高模拟底物所在环境黏附筛选培养目标组织工程皮肤种子毛囊细胞,具有高传代能力,高增殖能力,并可在体外环境下形成分化皮层结构。筛出免疫原性细胞安全获得免疫逃逸,延长移植时间增加组织工程临床应用,促进创面愈合与皮肤疾病提供了绝对优势临床治疗级细胞的解决方案。
The invention discloses culturing, screening and amplifying techniques for hair follicle stem cells for clinic treatment level cell therapy. The bottlenecks are always research hotspots and difficulties in the biological stem cell field; currently, by carrying out separation and purification by virtue of remarkable adhering properties of a basement membrane, the hair follicle stem cells are obtained by virtue of three-dimensional culture and amplification measures. By depending on amplification anchorage-dependent cells, a three-dimensional high-simulation in-vivo extracellular matrix system is provided for screening and separating the levels of target cells. The method is a novel culture technique for culture in vitro of biological cells of stem cells of adults (embryo skin) in a high-simulation in-vivo adhesion environment. Skin seed hair follicle cells of targeted tissue engineering cultured through adhesion screening in the environment of a high-simulation substrate have high abdication capacity and multiplication capacity and can form a differentiated cortex structure in vitro. By screening immunogenic cells, immune escape is safely achieved, the transplant time is prolonged, the clinic application of tissue engineering is increased, the healing of wounds is promoted, and a solution with absolute advantages for the clinic treatment level cell therapy is provided for diseases of skin.

Claims

Description

Topics

Download Full PDF Version (Non-Commercial Use)

Patent Citations (0)

    Publication numberPublication dateAssigneeTitle

NO-Patent Citations (0)

    Title

Cited By (0)

    Publication numberPublication dateAssigneeTitle