基于二肽的聚合物材料及其在糖分离和糖肽富集中的应用

Dipeptide based polymer materials and application of dipeptide based polymer materials in saccharide separation and glycopeptide enrichment

Abstract

本发明涉及一种对糖具有优异区分能力的二肽功能单体的筛选方法,构筑了基于标准偏离参数D和R值筛选后得到的二肽化合物,并进一步将筛选获得的二肽化合物与含双键的基团偶联,得到可聚合的二肽功能单体,将它们聚合到各种基体表面上,得到了一系列聚合物修饰的色谱固定相材料。该材料展现出对寡聚半乳糖,寡聚果糖,唾液酸衍生物优异的分离能力;同时该材料对于糖肽具有优异的选择性富集性能和对糖链结构的区分能力,同时具有很高的糖肽回收率和重复性,在翻译后修饰蛋白质组学研究等领域将具有广阔的应用前景。
The invention relates to a screening method of dipeptide functional monomers having excellent separating capacity on saccharide. A dipeptide compound obtained on the basis of screening of standard deviation parameter D and R values is constructed, the dipeptide compound obtained through screening is further coupled with a group containing double bonds, polymerizable dipeptide functional monomers are obtained and are polymerized on surfaces of various matrixes, and a series of polymer modified chromatographic stationary phase materials are obtained. The materials have excellent separating capacity on oligomeric galactose, oligomeric fructose and sialic acid derivatives; meanwhile, the materials have excellent selective enrichment performance on the glycopeptide and separating capacity on a carbohydrate chain structure, have very high glycopeptide recovery rate and repeatability and have the wide application prospect in the fields of posttranslational modification proteomics research and the like.

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